| FKN;CX3CL1

Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) is a protein encoded by the CX3CL1 gene. Fractalkine is a large cytokine protein of 373 amino acids that contains multiple domains and is the only known member of the CX3C chemokine family. The polypeptide structure of CX3CL1 differs from the typical structure of other chemokines. CX3CL1 is produced as a long protein with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	2.28	1.82	1.23	0.8	0.53	0.36	0.23	0.08
Corrected OD	2.2	1.74	1.15	0.72	0.45	0.28	0.15	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse FKN;CX3CL1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Mouse FKN;CX3CL1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108